Optimization of process parameters for bioproduction, isolation and purification of 4-Ipomeanol from cell suspension cultures of <i>Ipomoea batatas</i> (L.) Lam.

Abstract

The secondary metabolite, 4-Ipomeanol from Sweet potato (<i>Ipomoea batatas</i>) is a potential chemotherapeutic agent for cancer. This study presents an efficient protocol for bioproduction of 4-ipomeanol, in large quantities without disturbing the natural biodiversity of <i>I. batatas.</i> Friable Calli from the root tubers of <i>I. batatas </i> were used to establish cell suspension cultures in liquid media under agitated conditions and the growth and production kinetics of the same were monitored. The presence of 4-ipomeanol was confirmed by NMR and IR spectroscopy and quantification was done using HPLC. A maximum of 7.39 mg g^-1 of 4-ipomeanol was obtained under biotic elicitation of the cell suspension cultures. The elicitors were studied for optimization of the day of addition and maximum bioproduction of 4-ipomeanol was observed when elicitors were added to 4 day old suspension cultures. Further, the 4-ipomeanol thus produced demonstrated cytotoxicity against renal carcinoma cell lines.