Deprotonation energetics of disodium uridine 5'-monophosphate and disodium guanosine 5'-monophosphate in water from emf measurements

Abstract

The second step and third step deprotonation constants of 5'-uridine monophosphate and 5'-guanosine monophosphate have been obtained in water from emf measurements of Hamed Ehler-type cells comprising H₂ and Ag-AgIelectrodes at different temperatures. The <i>p</i>K values have been fitted in the temperature equation <i>p</i>K = AT^-1 + B + CT by least squares method and the related thermodynamic quantities viz., <img src='/image/spc_char/delta.gif' border=0><i>G</i>^o, T<img src='/image/spc_char/delta.gif' border=0>S^o and <img src='/image/spc_char/delta.gif' border=0><i>H</i>^o have been obtained using the values of the coefficients A, B and C of the respective mononucleotides.