Optimization of Cordycepin Extraction from <em>Cordyceps militaris </em>Fermentation Broth

Abstract

Cordyceps is one of the components of traditional Chinese medicine. This mushroom becomes worldwide famous due to its significant therapeutic effects on various diseases. Nucleoside like cordycepin, a major bioactive compound from Cordyceps militaris, is commonly known as promising anticancer and antitumor compound. The aim of study is to determine an optimal mixture ratio of solvent-solvent-based extraction method, which is used for extraction of cordycepin, from cultivated <em>Cordyceps militaris</em> DSMZ 23612 fermentation broth. Hence, the optimization process was performed using statistical (RSM) design leaded an optimal cordycepin extraction using solvent mixture including hot water (23%), ethanol (30%), methanol (25%) and ethyl acetate (22%). In addition, the final extracted cordycepin (786.94 mgL^-1 of 81% purity) was achieved through extraction parameters as sample: solvent ratio (v/v), extraction temperature, extraction time and frequency at (1:10), 35 &deg;C, 90 min and 1 cycle, respectively. The experimental values obtained were fit with those predicted, thus indicate the suitability of RSM and method for optimization of cordycepin extraction.