Conjugation, labeling and characterization of asparaginase bound silver nanoparticles for anticancer applications

Abstract

Nanomedicine in cancer therapy is an emerging technology over the commercially available drug delivery systems. The loss of drug activity can be prevented by conjugating asparaginase with metal nanoparticles. This necessitates the synthesis of metal nanobiocomposite of L-asparaginase labeled with fluorescein isothiocyanate for better diagnosis and treatment. The present work is focused on the synthesis of silver nanocomposite of fungal L-asparaginase labeled with fluorescein isothiocyanate. The formation of fluorescein isothiocyanate labeled silver nanobiocomposite of asparaginase was confirmed with a broad peak from 360 nm to 500 nm in UV spectrum. The functional group present in the labeled silver nanobiocomposite of asparaginase was analyzed using FTIR spectroscopy while the presence of asparaginase in labeled silver nanobiocomposite was confirmed by ¹H-NMR. The anticancer activity of labeled silver nanobiocomposite of asparaginase as revealed by the fluorescent microscope against MG-63 cancer cell line showed an IC50 value of 62.5 µg/mL.