Production and characterization of thermostable gelatinase from <i>Bacillus globisporus</i> isolated from Unkeshwar hot spring of Maharashtra

Abstract

An efficient thermostable gelatinase producer was isolated from Unkeshwar hot spring of Nanded district in Maharashtra. It was identified as <i>Bacillus globisporus</i> (APP11) by observing its morphological characters, microscopic features, biochemical pattern and physiological characterization. It showed luxuriant growth and thermostable gelatinase production at 50 ^oC temperature. Effect of various agro-industrial residues on gelatinase production was determined. Maximum gelatinase production (675 ± 6.16 U/mg) from APP11 isolate was recorded after 24 h of time period using fish scale powder in the medium. Partially purified APP11 gelatinase was remarkably active at pH range 6 to 9 and temperature range 35 to 65 ^oC. Km and Vmax values of partially purified APP11 gelatinase were 3.0 mg/mL and 1000 U/mg respectively. The APP11 originated gelatinase showed remarkable activity in presence of selected metal ions.