<em>Althaea rosea</em> L. ameliorates renal oxidative damage induced by gentamicin in rats

Abstract

<em>Althaea rosea </em>L<em>. </em>(AR) seeds are commonly used in renal disorders in Unani medicine. Hence, the present study was aimed to evaluate the nephroprotective activity of methanolic extract of AR seed (ME-AR) and its chloroform soluble (CHSF) and insoluble (CHIF) fractions in gentamicin-induced renal oxidative damage in Albino rats. The rats were divided into five groups of six animals each. Group I served as control. All five groups were received 1 % CMC (10 mL/kg), orally for 8 days. In addition to this, the groups III, IV and V were received ME-AR 103 mg/kg, CHSF 70 mg/kg, and CHIF 35 mg/kg respectively, orally for 8 days. Gentamicin was injected subcutaneously (100 mg/kg) in neck region in the volume of 1 mL/kg for the last 5 days for the group II (Toxicant), III, IV and V. Following treatment, the nephroprotective effects were evaluated with level of blood urea nitrogen (BUN), serum creatinine (SCr), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and lipid peroxidation (LPO) along with the histological observations among the experimental groups. Gentamicin intoxication induced changes of biochemical parameters were significantly prevented by test drug which was also evidenced by the histological observations.