Fibrinolytic effect of phyto-proteins on animals and poultry blood

Abstract

Phyto-proteins of <em>Mangifera</em> <em>indica</em> L.<em>, Aegle</em> <em>marmelos</em> (L.) Corr&ecirc;a<em>, </em><em>Volvariella volvacea </em>(<em>Paddy straw mushroom</em>),<em> Carica</em> <em>papaya</em> L., <em>Ocimum</em> <em>sanctum</em> L. and<em> Aloe</em> <em>vera</em> (L.) Burm. f. extracted in water and PBS under cold percolation are precipitated in 50% acetone and quantified after reconstitution. PBS suspension with higher protein is assayed for thrombolytic effect on blood clots of Bovine, Caprine, Canine and Avian species and the fibrinolytic effect on fibrin-agarose plate with streptokinase as positive control. Phyto-proteins exhibit clot lysis effect of 49.41-92.04% in poultry, 41.60-93.73% in cattle, 46.67-85.85% in goat and 34.39-77.09% in dog blood as compared to 100 % of streptokinase. In 150 min incubation, significantly higher (p &lt; 0.05) amount of clots are disintegrated by proteins of <em>M. indica</em> and <em>A. marmelos</em> in dog, <em>V. volvacea</em> in poultry and goat, <em>C. papaya </em>and <em>O. sanctum</em> in goat, dog and cattle and <em>A. vera</em> in goat blood as compared to rest species. Phyto-protein and streptokinase impregnated discs depict 74.05-274.49 mm² and 484.48 mm² fibrinolysis zones on fibrin-agarose plate, respectively. Proteins of <em>M. indica,</em> <em>A. vera</em> and <em>O. sanctum</em> exhibits &gt; 50% and those of <em>A. marmelos,</em> <em>V. volvacea</em> and <em>C. papaya</em> exhibits &lt; 50% lysis than streptokinase enzyme. Phyto-proteins pose variable fibrinolytic activity on clots of animals and poultry blood similar to streptokinase.