Development and characterization of microsatellite loci from mango (<em>Mangiferaindica</em> L.)

Abstract

In the present study, a microsatellite enriched partial genomic library was constructed for mango. Twenty sequence tagged microsatellite site loci were characterized by employing M13 tailed PCR technique. All the 20 microsatellite loci were found to be efficient in discriminating and identifying the 20 diverse cultivars of mango used in this study. The genetic analysis for 20 microsatlellite loci showed that expected heterozygosity ranged 0.659 to 0.965, with a mean of 0.892, and the observed heterozygosity ranged 0.350 to 0.850, with a mean of 0.505. The polymorphic information content values ranged 0.624 to 0.938, with a mean of 0.860. The probability of identity(PI) values ranged 0.012 to 0.182, with a mean of 0.050. The total PI was 1.06× 10^-28. These novel SSR markers could be further exploited for genetic diversity studies, cultivar identification, linkage map development and association studies.