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http://nopr.niscpr.res.in/handle/123456789/43319| Title: | Molecular cloning and in silico analysis of cellulasegene from Bacillus amyloliquefaciens MBAA3 |
| Authors: | Thakkar, Aarti Saraf, Meenu |
| Keywords: | Bacillusamyloliquefaciens;Bioinformatics;Cellulase gene;Cloning;DNA;ExPASy |
| Issue Date: | Jul-2017 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Studies have been able to determine the distribution and diversity of cellulase genes in soil populations of bacteria in the rhizosphere A novel isolate of B. amyloliquefaciens MBAA3 from the rhizosphere soil was used to isolate and purify the cellulase gene.The gene was amplified, sequenced and the amplified product was ligated into pCR™4-TOPOR. The vectors containing cellulase gene were transformed and expressed in E. coli for further characterization.The cloning of the gene was confirmed through blue white screening and colony PCR methods. The clone was confirmed for nucleotide analysis. Cellulase from B. amyloliquefaciens was analyzed using computational tools. The physicochemical properties of the cellulase were analyzed by using ExPASy’s ProtParam tool and the molecular weight was determined 36732.1da.Isoelectric points (pI) was found to be alkaline in nature was 7.21. The secondary structure prediction was done by SOPAMA software and a 3D structure of cellulase was obtained by (PS2): Protein structure prediction server. |
| Page(s): | 487-494 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.16(3) [July 2017] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 16(3) 487-494.pdf | 783.9 kB | Adobe PDF | View/Open |
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