Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/43334| Title: | In vitro propagation of Nyctanthes arbor-tristis L. |
| Authors: | Raja, H David Arockiasamy, D I |
| Keywords: | In vitro propagation;Nyctanthes arbor-tristis;Plant regeneration;Culture condition |
| Issue Date: | Jul-2017 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Mass propagation of tree species has always been a challenging task. An efficient protocol for rapid in vitro mass propagation of Nyctanthes arbor-tristis L. has been developed from shoot tip and internodal explants. Explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylamino purine (BAP) and thidiazuron (TDZ) in various concentrations. 2. 0 mg/l BAP was proved to be best for high frequency multiple shoot induction from shoot-tip explants and 2. 5 mg/l BAP was produced maximum shoots proliferation from internodal explants. The well elongated healthy shoots were transferred for rooting on MS medium supplemented with indole-3-butyric acid (IBA) in different concentration. Maximum numbers of roots were achieved in 2.0 mg/l of IBA medium. The rooted plantlets were hardened and transferred to field conditions. All the regenerated plants with 80-85% survival rate were normal with respect to morphology and growth characteristics. |
| Page(s): | 366-369 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.16(3) [July 2017] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 16(3) 366-369.pdf | 265.85 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.