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Title: Genetic polymorphism at exon-2 MHC B-LβII family gene by PCR-SSP in Kadaknath chicken
Authors: Thakur, Mohan Singh
Parmar, Shivnandan Singh
Kumar, Amit
Keywords: Genetic polymorphism;MHC gene;PCR-SSP;Kadaknath chicken
Issue Date: Jul-2017
Publisher: NISCAIR-CSIR, India
Abstract: The present study was conducted to find out the MHC B-Lβ family (class II) alleles polymorphism in Kadaknath chicken using polymerse chain reaction-sequence specific primer (PCR-SSP). Blood samples were collected from each of the birds and genomic DNA was isolated. A set of degenerating primers was used to amplify a 235 bp, exon-2 region of chicken MHC B-Lβ family allele. The products of the first round of amplification were subjected to PCR-SSP at the specified annealing temperatures and replication cycles. The primers designated for B2, B13, B15 and B19 amplified the target sequences produced fragments of 222 bp, 141 bp, 222 bp and 213 bp sizes, , respectively. None of the samples of Kadaknath chicken was amplified with primers designed to target B21 allele. Thus, 8 different genotypes were identified in Kadaknath chicken. It was observed that the allele B2 and B13 exhibited predominance while genotypic distribution revealed abundance of B2B13 followed by B2B15 and B2B2 in the of Kadaknath birds population under study.
Page(s): 329-332
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.16(3) [July 2017]

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