<em>Streptomyces castaneoglobisporus</em> AJ9, A haloalkaliphilic actinomycetes isolated from solar salt works in southern India and its pharmacological properties

Abstract

Five strains of haloalkaliphilic <em>Streptomyces </em> sp<em>.</em> were isolated and identified. Among the five strains, the <em>Streptomyces</em> sp. AJ9 which isolated Kovalam solar salt works had the potential ability of protease, gelatinase enzymes production and highly antagonistic against the pathogenic bacteria including <em>Aeromonas hydrophila, Escherichia coli, Salmonella typhi, Staphylococcus aureus</em> and <em>Vibrio parahaemolyticus. </em> The <em>Streptomyces</em> sp. AJ9 was identified by genomic level through 16S rRNA sequencing and confirmed as <em>Streptomyces</em> <em>castaneoglobisporus</em> AJ9 (GenBank KC603900.1). Further the secondary metabolites were extracted with ethyl acetate, purified through silica column chromatography and screened the fractions (FI to FVII) against pathogenic bacteria, fungi, the shrimp killer virus, White Spot Syndrome Virus (WSSV). Among the fractions FIV to FVI had highest bioactivities and the pooled fractions were structurally characterized by GC-MS analysis and confirmed as the benzoic acid derivatives of 2-hydroxy-4-methoxy-6-methyl-, methyl ester. The pooled fraction (FIV to FVI) were significantly (P<0.001) controlled the bacterial, fungal and viral pathogens by <em>in vitro</em> levels and able to kill the L 929 cancer cells at the rate of 75.58 % µg/ml.