Urease immobilized potentiometric biosensor for determination of urea

Abstract

A simple potentiometric biosensor for the determination of urea is reported in this work. The biosensor has been constructed by immobilization of urease at tungsten electrode using polyvinyl alcohol matrix followed by the sealing process with plasticised polyvinyl chloride. The sensing device gives fast and sensitive response to urea. A linear calibration curve in the concentration range of 0.01–50 mM urea is obtained with slope 42.7 mV per decade urea concentration (<em>r</em>² = 0.993), and detection a limit of 0.01 mM urea.