An Approach to Identify Sterol Entities from <em>Abrus Precatorius</em>’s Seeds by GC-MS

Abstract

Sterols are most important compounds in living organisms and it is found in unsaponifiable fraction of plant. The objective of this investigation was to identify the chemical constituents of the seeds of <em>Abrus Precatorius.</em> A plant used for medicinal purpose in folklore. Petroleum ether extract of the seeds was used for this study. Saponifiable fraction and unsaponifiable fractions were separated. Compounds extracted in di-ethyl ether and separated using column chromatography. Stigmasterol, gamma sitosterol, campesterol were identified from the seeds by using gradient elution. The eluted chemical compounds were analysed by GC-MS. The present analysis revealed that seeds of <em>Abrus Precatorius</em> contain three sterols compound. Principal themes of the study were to highlight the development and application of chromatographic techniques for the detection of the sterols compounds by single injection which will be used for natural product and pharmaceutical industries.