Anti-cervical cancer and anti-metastatic effects of sub-fraction 1a of <em>Solanum nigrum</em> L.

Abstract

In the present study, the crude polyssaccharides from <em>Solanum nigrum</em> L. was fractioned by DEAE-cellulose column chromatography, and was further purified by Sephadex-100 column chromatography. We investigated the anti-cervical cancer activity and anti-metastasis effects of the sub-fraction 1a of polysaccharides from <em>Solanum nigrum</em> L. (SNL-P1a) in tumor-bearing mice models, and explored the probable mechanism underlying the pharmacological activity of the polysaccharide. Mice were grouped into the model control, positive control and SNL-P1a of low and high dose treatment groups. After experiment ended, the tumor growth and the lung metastasis inhibition rates were calculated respectively. Furthermore, the serum antioxidant enzyme activities in mice were measured by spectrophotometer method. Results showed that SNL-P1a inhibited the growth and the metastasis of cervical cancer significantly. Moreover, SNL-P1a treatment increased serum antioxidant enzyme activity and LDH activity. These results suggested that the antioxidant activity of SNL-P1a might be beneficial to the cervical cancer therapy.