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http://nopr.niscpr.res.in/handle/123456789/44811Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Sharma, Shruti | - |
| dc.contributor.author | Kumar, Ashok | - |
| dc.contributor.author | Dobhal, Shivani | - |
| dc.contributor.author | Rawat, Anita | - |
| dc.date.accessioned | 2018-08-07T04:20:57Z | - |
| dc.date.available | 2018-08-07T04:20:57Z | - |
| dc.date.issued | 2018-01 | - |
| dc.identifier.issn | 0975-0967 (Online); 0972-5849 (Print) | - |
| dc.identifier.uri | http://nopr.niscair.res.in/handle/123456789/44811 | - |
| dc.description | 185-188 | en_US |
| dc.description.abstract | A simple, rapid and efficient method for extraction of genomic DNA from young leaves of Melia composita Benth. (Syn. M dubia Cav.) was optimized for obtaining fine quality DNA. The yield of extracted DNA ranged from 118.40 to 3192.40 µg /500 mg of tissue with absorbance ratio (A260/A280) ranging from 1.41 to 1.96. The quality of extracted DNA was also evaluated by agarose gel electrophoresis which showed a single intact band. The isolated DNA was free of any contaminating proteins, polysaccharides and coloured pigments and successfully amplify inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJBT Vol.17(1) [January 2018] | en_US |
| dc.subject | Melia composite | en_US |
| dc.subject | DNA extraction | en_US |
| dc.subject | Inter simple sequence repeat (ISSR) | en_US |
| dc.subject | Simple sequence repeat (SSR) | en_US |
| dc.title | Standardization of protocol for genomic DNA isolation in Melia composita Benth. (S. M. dubia Cav.) | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJBT Vol.17(1) [January 2018] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 17(1) 185-188.pdf | 681.54 kB | Adobe PDF | View/Open |
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