Developing micropropagation protocol and analyzing peroxidase activity during morphogenesis in <em>Arisaema decipiens</em> Schott, a medicinal plant

Abstract

An efficient micropropagation system for <em>Arisaema decipiens</em> Schott, an ethnic medicinal plant, has been developed. Calluses were induced from petiole explants on Murashige and Skoog (MS) medium supplemented with 0.5 mg l^-1 N⁶-benzyladenine (BA) and 1.0 mg l^-1 2,4-dicholorophenoxyacetic acid (2,4-D). The effect of 1.0 mg l^-1 2,4-D in combination with 1.0 mg l^-1 BA was much more pronounced for the callus proliferation, and fresh weight of callus increased 11.72 times after 5 week of culture. Significantly more protocorm-like bodies (PLBs) could be obtained on calluses exposed to 0.5 mg l^-1 indole-3-acetic acid (IAA) in combination with 0.5 mg l^-1 BA. In this medium, the PLBs transformed into shoots, and frequency of shoot induction was about 82%. Up to 100% of the regenerated shoots formed complete plantlets on MS medium supplemented with 0.5 mg l^-1 IAA in combination with 0.5 mg l^-1 BA, with an average of 10.35 roots and 7.43 cm long per shoot. Peroxidase (POD) expressions were measured by polyacrylamide gel electrophoresis during shoot induction and differentiation phases. The results showed that POD activity was connected with the different states of shoot morphogenesis.