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http://nopr.niscpr.res.in/handle/123456789/45112Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Lal, R | - |
| dc.contributor.author | Soni, K B | - |
| dc.contributor.author | Alex, S | - |
| dc.contributor.author | Sreekantan, L | - |
| dc.contributor.author | Reghunath, B R | - |
| dc.date.accessioned | 2018-10-05T04:41:54Z | - |
| dc.date.available | 2018-10-05T04:41:54Z | - |
| dc.date.issued | 2018-10 | - |
| dc.identifier.issn | 0975-1084 (Online); 0022-4456 (Print) | - |
| dc.identifier.uri | http://nopr.niscair.res.in/handle/123456789/45112 | - |
| dc.description | 583-586 | en_US |
| dc.description.abstract | Metagenomic DNA was isolated from dairy effluent collected from a milk processing unit. The Hpa I digests of metagenome were cloned in pEZ BAC vector. The transformed DH5 colonies were selected by blue white screening on chloramphenicol (25μg/ml) containing plates. Functional screening of the library on skim milk agar showed a clone having protease activity. This clone was confirmed to have a metagenome insert of size approx. 9kb. The crude cell extract of this clone at 24th h of growth showed protease activity of 166.99U/ml (111.32U/mg protein). The protease was found to be alkaline and had maximum activity at 400C and pH 9.0. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | JSIR Vol.77(10) [October 2018] | en_US |
| dc.subject | Metagenome | en_US |
| dc.subject | DNA | en_US |
| dc.subject | Transformed | en_US |
| dc.title | Identification and Characterization of a Protease Encoding Metagenome Clone from Dairy Effluent | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | JSIR Vol.77(10) [October 2018] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 77(10) 583-586.pdf | 192.89 kB | Adobe PDF | View/Open |
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