Antimutagenic activity of methanolic extracts of four ayurvedic medicinal plants

Abstract

Methanolic extracts of <i style="">Acorus calamus</i> (Rhizome), <i style="">Hemidesmus indicus</i> (Stem), <i style="">Holarrhena antidysenterica</i> (Bark) and <i style="">Plumbago zeylanica</i> (Root), were tested for their antimutagenic potential. These extracts, at tested concentrations, showed no sign of mutagenicity to <i style="">Salmonella typhimurium</i> tester strains. The extracts of the plants exhibited varying level of antimutagenicity. At a dose of 100 µg/plate, the extracts exhibited the inhibition of His+ revertants from 18.51% to 82.66 % against direct acting mutagens, methyl methanesulphonate (MMS) and sodium azide (NaN₃) induced mutagenicity in <i style="">Salmonella</i> tester strains TA 97a, TA 100, TA 102 and TA 104. However, at lower concentrations (25 and 50 µg/ plate) of the plant extracts, a decrease in antimutagenic activity was recorded. Dose dependent antimutagenic activity of the extracts is also evident from linear regression analysis of the data. The over all antimutagenic potential of above four extracts was found to be in order of <i style="">A. calamus </i>> <i style="">H. indicus</i> > <i style="">H. antidysenterica</i> > <i style="">P. zeylanica</i>. Further, total phenolic content of these extracts did not correlate with its antimutagenic activity in <i style="">A. calamus</i> and <i style="">P. zeylanica</i>.