Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/4651| Title: | Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles |
| Authors: | Hema, M Reddy, Ch V Subba Savithri, H S Sreenivasulu, P |
| Keywords: | Potyvirus-like particles;Recombinant coat protein;Sugarcane streak mosaic virus |
| Issue Date: | Nov-2008 |
| Publisher: | CSIR |
| Abstract: | Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization. |
| Page(s): | 793-796 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.46(11) [November 2008] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 46(11) 793-796.pdf | 316.79 kB | Adobe PDF | View/Open |
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