Determination of antioxidant, antimicrobial and antitumor activity of bryophytes from Mount Ida (Canakkale, Turkey)

Abstract

In this study, active components of bryophytes were extracted from the samples collected from Mount Ida (Canakkale- Turkey). Three different extraction methods (Soxhlet, supercritical fluid extraction-SFE, Soxhlet after SFE) were employed for 2016 samples while direct Soxhlet extraction was carried out for 2017 samples using methanol as extracting solvent. Initially, antioxidant activity tests of the crude extracts were performed for all the samples. Antioxidant properties were determined to be fairly weak or moderate in the majority of the species studied. <em>Marchantia polymorpha</em> (T1), a liverwort, showed high extraction yield and antioxidant activity. Similarly, high activity was detected in <em>Hypnum cupressiforme</em> (T9) and <em>Neckera complanata</em> (T10) species. The antioxidant capacity of the tested species often sheds light on other activities. Antimicrobial properties were also tested for all extracts obtained in 2017. <em>Thuidium tamariscinum </em>(T8) was positive against<em> Acinetobacter haemolyticus </em>ATCC 19002 bactericin; T1 and <em>Isothceum myurum</em> (T11) were positive against <em>Bacillus subtilis TCC6633</em> bacteria and all other species were negative. Following these results, we have concentrated on the most active species and carried out antitumoral tests. Again, fractioned polar isolate of T1 was the only antiproliferative species against HeLa and A549 lung cancer cells.