Phytochemical analysis, in vitro antioxidant activity and anti-inflammatory activity of Turbinaria ornata in human RBC model

Abstract

<em>Turbinaria ornata </em>methanolic extract (TOME) with more bioactive compounds has been investigated for its <em>in vitro</em> total antioxidant activity, DPPH scavenging assay, reducing power assay, and antioxidant potential. Anti-hemolysis and anti-inflammatory activities were studied in human RBC model. Our preliminary phytochemical analysis showed that TOME constitutes carbohydrates, alkaloids, saponins, phenolic compounds, flavonoids, tannins, coumarines, steroids, and terpenoids. TOME at the concentration of 100 µg showed 89.11% of total antioxidant activity. The free radicals NO, H₂O₂ and SOD scavenging activities were enhanced with an increase in the concentration of TOME. Further, TOME at selected 0.5, 0.75 and 1(mg/ml) concentrations showed significant reduction in H₂O₂-induced hemolysis. The higher dose of 500 µg/ml (among selected concentrations) showed about 81 % of anti-inflammatory activity through enhanced stabilization of RBC membrane which was significant as the positive control diclofenac sodium. Thus our study clearly elucidated that <em>Turbinaria ornata </em>with potent bioactivities inhibited free radicals assault and prevented inflammation in RBC model.