Isolation of symbiotic bacteria and bioactive proteins from the marine sponge, <i style="">Callyspongia</i> <i style="">diffusa</i>

Abstract

The marine sponge, <i style="">Callyspongia</i> <i style="">diffusa</i>, collected near Mumbai coast, was studied for symbiotic bacterial association and biologically active proteins. Four bacterial species<i style=""> </i>(<i style="">Pseudomonas aeruginosa</i>, <i style="">Escherichia coli</i>, <i style="">Vibrio parahaemolyticus</i> and <i style="">V. cholera</i>)<i style=""> </i>were found associated with the sponge. Crude protein from the sponge was extracted at concentration of 1.43 mg/mL in methanol and 1.62 mg/mL in aqueous extract. Antibacterial activity of the extracts against the four symbiotic bacteria inhibited the growth of only <i style="">V. cholera</i>. Both extracts exhibited hemolytic activity on chicken erythrocytes at 6.99 HT/mg for methanolic and 8.64 HT/mg for aqueous extracts. On SDS-PAGE, the crude protein yielded eight bands in methanolic extract and five bands in aqueous extract, with molecular weight ranging from 14.4 to 116 kDa with three well defined bands of 19.5, 39.0, 66.2 kDa in both the extracts.