Enhanced alpha-amylase production using Streptomyces gancidicus ASD by process optimization

Abstract

The present study was focused on purification and optimization of amylase from marine actinomycetes. Among 101 actinomycetes isolates from Andaman & Nicobar islands, <em>Streptomyces sp gancidicus_ASD</em> was isolated and further studied. The enzyme activity was studied at various physical parameters like temperature, pH, carbon source, Nitrogen source, metal ions, NaCl concentration etc. by maintaining all the factors with 100 ml of crude extract. Also, media optimization with response surface methodology was used to ameliorate the bioprocess economics. A central composite design was conducted to optimize the four selected factors. Statistical analyses of the data of model fitting were done by using Design expert 10.0 (stat-Ease). Results show a maximum predicted amylase yield of 11460.34 IU/ml when using 1.05% sucrose, 0.608% beef extract, 7.1 pH and 40.35 °C temperature. The predicted value is approximately 1.24-fold much higher than the original production (9248 IU/mL) determined by the conventional one-factor-at-a-time optimization method which can be applied in bioprocess for increased amylase yield.