Regulation of Process Parameters for Improved Synthesis of Thrombolytic Enzyme from Bacillus cereus S46

Abstract

The study aimed to isolate soil bacteria and screen them for thrombolytic activity. Effect of process parameters on enzyme production were analyzed using one factor at a time approach. Isolate S46 demonstrated highest blood clot dissolution (15.64%) and was chosen for further study. Based on the partial 16S rDNA sequence, isolate S46 was identified as <em>Bacillus cereus</em>. Addition of sucrose and casein (1% w/v) to the broth resulted in highest enzyme yield (130 U/ml). Enzyme production improved in presence of MgSO₄ (0.08%, w/v), FeSO₄ (0.004% w/v), respectively. Enzyme production was best (186.66 and 210.86 U/ml) at pH 8.0 and 30 °C. Maximum enzyme production (220.86 U/ml) was reported at 72 h with inoculum size of 1.5% v/v. The above conditions advocate that media optimization enhanced the thrombolytic enzyme yield by <em>Bacillus cereus</em> S46.