E. coli AB 1157 susceptibility test, MTT assay on MCF-7 and HeLa cell lines of root and leaf fractions of Viburnum Linn. species

Abstract

The aim of this study was to evaluate mutagenic bacterial susceptibility and cytotoxic potency of <em>Viburnum coriaceum</em> Blume and <em>Viburnum erubescens</em> Wall.ex DC in order to report the actual chemotherapeutic potentials of these two species. The methanolic (80%) leaf and chloroform root extracts of <em>Viburnum </em>Linn. Species were tested for their bacterial strain based cytotoxicity employing Agar diffusion method suing <em>E. coli </em>AB 1157 strain. Also, the MTT assay was carried out employing MCF-7 breast cancer cell lines and HeLa cervical cell lines. It was started with IC₅₀ value determination of the selected test extracts from the results of bacterial strain based cytotoxicity. Upon increase in concentration up to 1000 µg/mL in agar diffusion cytotoxicity studies VCMLE, VEMLE and VCCRE had shown diameter of inhibition zone 10 mm, 9 mm and 10 mm respectively. Among other extracts, the VEMLE and VCCRE were selected to go ahead with anticancer activity by MTT assay. The potentials of extracts through cytotoxicity mechanism had produced 30-40% protection against cancer cell lines. It was concluded that VEMLE and VCCRE had produced the cytotoxic effect on <em>E. coli</em> AB 1157 strain. and were selected for the cytotoxic studies. The effects exhibited by the selected extracts may be due to the presence of diverse number of active constituents present in <em>Viburnum </em>Linn species also may be to the presence of unreported constituents.