Genetic polymorphism in Dalbergia sissoo Roxb. using RAPD markers

Abstract

Genetic diversity in forty nine genotypes of <em>Dalbergia sissoo </em>Roxb. was analyzed using randomly amplified polymorphic DNA (RAPD) with 10 decamer primers. A total of 129 distinct DNA fragments (bands) were amplified, of which 109 bands were polymorphic ranged from 200 to 1300 base pair. In fact, the number of amplified bands per genotypes varied from 7 to 14 and polymorphism percentage ranged from 63.63 to 93.33 with an average of 84.93. The mean polymorphic information content (PIC) for RAPD primers was recorded to be 0.24 which ranged from 0.24 to 0.33. The Jaccard similarity coefficient ranged from 0.62 to 0.93 showing wide range of variability among the genotypes. The unweighted pair group method with arithmetic averages (UPGMA) and principle coordinate analysis (PCoA) depicted relationship among the genotypes, which were found to be complimentary to each other. The maximum genetic diversity (0.38) was reported between genotypes FRI/DS/7006 and FRI/DS/0059, whereas genotypes FRI/DS/0218 and FRI/DS/1003 were found to be least divergent (0.07). The genotypes were grouped into eight clusters, off which, cluster II contained maximum twenty genotypes, and cluster VII and VIII comprised just one genotypes each.