<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Expression of an antimicrobial peptide (MSI-99) confers enhanced resistance to <i style="">Aspergillus niger </i>in transgenic potato<i style=""></i> </smarttagtype></smarttagtype>

Abstract

<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> MSI-99, a synthetic substitution analogue of antimicrobial peptide magainin, was used to impart enhanced resistance against fungal pathogen, <i style="">Aspergillus niger</i> in transgenic potato cultivars, Kufri Jyothi and Kufri Bahar.<i style=""> </i>Internodal stem segments were transformed with <i style="">Agrobacterium tumefaciens</i> strain EHA105. Integration of the transgene, MSI-99 in the transgenic plants of both the cultivars was confirmed by PCR and PCR-Southern analysis, exhibiting 180 bp fragment and hybridization with labelled probe pSAN168. Bioassay studies using detached leaves of transgenics showed the enhanced resistance against <i style="">A. niger</i>. </smarttagtype></smarttagtype>