Standardization of <i style="">in vitro</i> protocol in <i style="">Chrysanthemum</i> cv. Madam E Roger for development of quality planting material and to induce genetic variability using <img src='/image/spc_char/gamma2.gif'>-radiation

Abstract

‘Madam E Roger’, a greenish white large flowered chrysanthemum cultivar- was selected for <i style="">in vitro</i> propagation and mutagenesis to induce further genetic variability. A protocol has been standardized to develop large-scale quality planting material for commercial exploitation. Shoot bud differentiation could be achieved in ray florets in the presence of 0.5 mg L^-1 NAA+ 2.0 mg L^-1 BA. The differentiated shoots were further proliferated in the same medium, rooted in the presence of 0.5 mg L^-1 NAA and hardened to grow in the field. Genetic variability in the form of a solid mutant of yellow colour has been obtained from this cultivar, when the freshly inoculated ray florets were treated with 1.0 Gy <img src='/image/spc_char/gamma2.gif'>-radiation dose.