Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/50511
Title: Binary immobilization: a newer approach for immobilizing lipase from a thermophilic sp. of Thermomyces lanuginosus
Authors: Gupta, Pritesh
Nipunta
Dutt, Kakoli
Saran, Saurabh
Saxena, Rajendra Kumar
Keywords: Binary;Chitosan;Immobilization;Reusability;Thermomyces lanuginosus lipase;Thermostability
Issue Date: Oct-2019
Publisher: NISCAIR-CSIR, India
Abstract: We report binary immobilization of Thermomyces lanuginosus lipase enzyme using chitosan as the support. This method of enzyme immobilization is better than cross-linked enzyme aggregate (CLEA) in terms of better enzyme recovery and separation. This method of immobilization resulted in an increase in the thermostability of the binary immobilized lipase as against the crude free enzyme. This preparation could be used for nearly 15 consecutive cycles with 80-100% efficiency. Reusability of the immobilized enzyme makes it an economical alternative to the traditional way. Immobilized lipases in particular are a modern catalytic tool for various industrially significant reactions and applications.
Page(s): 358-362
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.56(5) [October 2019]

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