Fabrication of chitosan-alginate microencapsulated curcumin coated scaffold to develop novel cotton crepe bandage

Abstract

Medicated cotton crepe bandages have been developed, using natural curcumin conjugated polymer nanoparticle with biocompatible and biodegradable properties. Curcumin (C) has been incorporated into Chitosan (Ch) and sodium alginate (A) microcapsule, which serve as templates for nanofibres. The coating of C-ChA results in chitosan scaffold (CS) containing curcumin-chitosan microcomplex (CS-CChM). Both the substances act as a powerful biomaterial for tissue engineering applications, especially for wound healing. CS-CChM along with C-ChA have been characterized using FTIR and SEM. The free radical scavenging effect is determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) and anti-inflammatory assay. The developed CS-CChM nanofibre shows an impressive mechanical stability which is an essential requirement for wound healing. The bearing of curcumin shows cytotoxic effect. The <em>in vitro </em>radical scavenging activity and the anti-inflammatory assay are performed by inhibition of albumin denaturation. These microencapsulated matrix displays potent anticancer activity against mouse fibroblast on both normal (L929) and human lung adenocarcinoma (A549) cells. The cell viability is observed to be 51.4 ± 0.71 and 30.53 ± 0.99 respectively for both. CS-CChM nanofibre is found to be stable against enzymatic degradation, which is the most important parameter for promoting the proliferation of cells, contributing to repair and remodeling of tissues during wound healing applications.