<i style="">In vitro</i> propagation of tikhur (<i style="">Curcuma angustifolia</i> Roxb.): A starch yielding plant

Abstract

<i style="">In vitro</i> regeneration of <i style="">Curcuma angustifolia</i> Roxb. was achieved through shoot meristem culture<i style="">. </i>The shoot buds (2-3 cm long) from rhizome were inoculated on MS medium supplemented with 3.0 mg/L BAP for initiation and elongation of shoots. As a result, 1.87±0.28 shoots per explant were produced These shoots were transferred on MS medium supplemented with 3.0 mg/L BAP and 25 mg/L adenine sulfate for further shoot multiplication. About 6.9±0.69 micro-shoots per explant were produced with in 6 wk. The roots appeared from shoots on shoot establishment as well as multiplication media. The rooted plants were transferred to pots and acclimatized, which showed 83% survival with normal growth.<b style=""></b>