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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Ranjitha, K | - |
| dc.contributor.author | Kaushik, B D | - |
| dc.date.accessioned | 2009-07-03T14:03:43Z | - |
| dc.date.available | 2009-07-03T14:03:43Z | - |
| dc.date.issued | 2005-05 | - |
| dc.identifier.issn | 0975-1084 (Online); 0022-4456 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/5141 | - |
| dc.description | 372-375 | en_US |
| dc.description.abstract | Purification of phycobiliproteins from phycoerythrin (PE) rich strain of Nostoc muscorum was carried out. Salting out with 55 % (NH4)2SO4 precipitates >80 % of each class of phycobiliprotein. Chromatography on DEAE cellulose-52 column gives pure PE (A562/A280 = 8.12), while rechromatography of the blue coloured fraction from this column using a hydroxy apatite column yields pure phycocyanin (PC) (A615/A280 = 3.89). SDS-PAGE analysis of purified PE and PC yields two polypeptides (~19.4 and 16.9 kDa). PE is found to be stable between pH 5-9. This protocol helps to get highly pure PE (72%) and PC (39%). | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | CSIR | en_US |
| dc.relation.ispartofseries | C 12 N 1/12 | en_US |
| dc.source | JSIR Vol.64(05) [May 2005] | en_US |
| dc.subject | Nostoc muscorum | en_US |
| dc.subject | Phycobiliproteins | en_US |
| dc.subject | Phycoerythrin | en_US |
| dc.subject | Phycocyanin | en_US |
| dc.subject | Allophycocyanin | en_US |
| dc.title | Purification of phycobiliproteins from Nostoc muscorum | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | JSIR Vol.64(05) [May 2005] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 64(5) 372-375.pdf | 209.47 kB | Adobe PDF | View/Open |
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