<i style="">In vitro </i>antioxidant studies in leaves of<i style=""> Annona </i>species<i style=""></i>

Abstract

Antioxidant potential of leaves of three different species of <i style="">Annona</i> was studied by using different <i style="">in vitro</i> models eg., 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS), nitric oxide, superoxide, hydroxy radical and lipid peroxidation. The ethanolic extract of <i style="">A.muricata</i> at 500 μg/ml showed maximum scavenging activity (90.05%) of ABTS radical cation followed by the scavenging of hydroxyl radical (85.88%) and nitric oxide (72.60%) at the same concentration. However, the extract showed only moderate lipid peroxidation inhibition activity. In contrast, the extract of <i style="">A.reticulata</i> showed better activity in quenching DPPH (89.37%) and superoxide radical (80.88%) respectively. <i style="">A.squamosa</i> extract<i style=""> </i>exhibited least inhibition in all <i style="">in vitro </i>antioxidant models excepting hydroxyl radical (79.79%). These findings suggest that the extracts of <i style="">A. muricata</i> possess potent <i style="">in vitro</i> antioxidant activity as compared to leaves of <i style="">A. squamosa </i>and <i style="">A.reticulata</i> suggesting its role as an effective free radical scavenger, augmenting its therapeutic value.