Cell adhesion by aqueous extract of human placenta used as wound healer

Abstract

Aqueous extract of human placenta, used as wound healer, has shown significant cell adhesion property on mouse peritoneal macrophages and P_388D1cultured macrophage cell line. This property was offered primarily by fibronectin type III like peptide present in the extract and is comparable to fibronectin on a molar basis. The peptide induce adhesion of cell through cell surface receptors having K_d = 2.8±0.9 x 10^-5 <i style="">M</i> suggesting weak binding. This is in support of integrins receptors that typically exhibit low affinities. Cell adhesion was partially inhibited by Arg-Gly-Asp (RGD) peptide, anti-β₁integrin suggesting that integrin β₁receptors have roles to play in the process.