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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Josephrajkumar, A | - |
| dc.contributor.author | Chakrabarty, R | - |
| dc.contributor.author | Thomas, G | - |
| dc.date.accessioned | 2009-07-20T10:10:56Z | - |
| dc.date.available | 2009-07-20T10:10:56Z | - |
| dc.date.issued | 2007-11 | - |
| dc.identifier.issn | 0975-1009 (Online); 0019-5189 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/5359 | - |
| dc.description | 998-1002 | en_US |
| dc.description.abstract | An elastase-like chymotrypsin was purified by aprotinin-agarose affinity chromatography from the midgut extract of cardamom shoot and capsule borer, Conogethes punctiferalis. The purified enzyme had a Vmax of 687.6 ± 22.1 nmole pNA released/min/mg protein, Km of 0.168 ± 0.012 mM with SAAPLpNA as substrate and gave a single band on SDS-PAGE with a molecular mass of 72.1 kDa. Casein zymogram revealed one clear zone of proteolytic activity, which corresponded to the band obtained with SDS-PAGE indicating that this could be a single-polypeptide enzyme. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | CSIR | en_US |
| dc.source | IJEB Vol.45(11) [November 2007] | en_US |
| dc.subject | Aprotinin | en_US |
| dc.subject | Cardamom | en_US |
| dc.subject | Chymotrypsin | en_US |
| dc.subject | Conogethes punctiferalis | en_US |
| dc.subject | Trypsin | en_US |
| dc.title | Purification of elastase-like chymotrypsin from cardamom shoot and Capsule borer | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJEB Vol.45(11) [November 2007] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 45(11) 998-1002.pdf | 206.56 kB | Adobe PDF | View/Open |
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