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http://nopr.niscpr.res.in/handle/123456789/55164| Title: | Development of a dual-gene PCR assay for simultaneous amplification of structural and non-structural genes of bovine rotavirus A |
| Authors: | Gupta, Vandana Nayak, Anju Swamy, Madhu Singh, R. V. |
| Keywords: | Bovine rotavirus;Dual-gene PCR;Structural gene;Non-structural gene diagnosis |
| Issue Date: | Jan-2020 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Rotavirus can be detected in stool samples by using serological and molecular methods. An easy and rapid dual gene RT-PCR assay for the simultaneous amplification of the capsid protein VP4 (structural) and rotavirus enter toxin NSP4 (non-structural protein) has been developed. We validated the assay by comparing the results of single target PCR with dual-gene PCR products. |
| Page(s): | 45-48 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.19(1) [January 2020] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 19(1) 45-48.pdf | 62.5 kB | Adobe PDF | View/Open |
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