In Vitro antimicrobial, antioxidant activity and phytochemical screening of Paeonia emodi

Abstract

This is the first scientific report to explore the comprehensive antimicrobial activity, antioxidant activity and phytochemical screening of different fractions (petroleum ether, ethyl acetate, methanolic and water) of leaves part of <em>P. emodi</em>. The extraction process involved fractionation with various solvents with using AOAC method and standard techniques. Antioxidant activities of methanolic extract were examined using DPPH methods. The level of starch, sucrose and malic acid <em>P. emodi</em> leaves were found to be 9.5%, 5.4% and 0.47%, respectively. The methanolic extract of <em>P. emodi</em> leaves showed the highest activity as compared with other plants. The antimicrobial activities were tested by the disc-diffusion method. Phytochemical screening of methanolic extract leads to identification of tannins, flavonoids, polyphenols, sterols, polyterpenes and alkaloids. The present study was attempted for the first time to investigate the antimicrobial activity, antioxidant activity, thin layer chromatography and phytochemical screening of <em>P. emodi </em>to search for newer, safer and more potent drug.