Biolistic-mediated DNA delivery and transient expression of GUS in hypocotyls of <i style="">Feronia limonia</i> L.—A fruit tree

Abstract

<i style="">Feronia limonia</i> L. (Rutaceae) is an important fruit tree of arid horticulture. To be able to introduce genes of interest for its improvement, biolistic-mediated DNA delivery method has been standardized. The hypocotyls explants were bombarded with plasmid pBI121 having <i style="">gus</i> reporter gene driven by CaMV 35S promoter and <i style="">npt</i> II under control of <i style="">nos</i> promoter as selectable marker. The best transient expression of GUS was recorded when tungsten particles coated with DNA were bombarded at a disc pressure of 1350 and 1100 psi from a distance of 9 cm. Some of the bombarded explants are being maintained to obtain viable regenerates for their analyses.