Production of PHB (bioplastics) using bio-effluent as substrate by <i style="">Alcaligens eutrophus</i>

Abstract

<i style="">Alcaligenes eutrophus</i> MTCC 1285 produced bioplastics (PHB) in N₂ deficient medium containing carbon (glucose concentration 2%). Using sago effluent, thippi and molasses as substrates and at different <i style="">p</i>H (6.9-8.0) the production of PHB was recorded; higher production of the biopolymer was obtained at <i style="">p</i>H 8.0 in the molasses based production media. The amount of PHB produced at <i style="">p</i>H 6.9 was 0.8, 0.5, 0.4, and 0.73 (g/mL^-1) and 1.1, 0.65, 0.55 and 1.0 (g/mL^-1), respectively in glucose, sago, thippi, molasses substrate based media. The present investigation revealed that bio effluents could be used for the production of PHB so as to decrease the pollution caused by them.