Identification of new strain of mungbean yellow mosaic virus infecting french bean (Phaseolus vulgaris L.) in Santiniketan

Abstract

Outbreak of yellow mosaic diseases emerged during 2017 in French bean (<em>Phaseolus vulgaris </em>L.) growing region of Santiniketan, Bolpur, West Bengal, India. Yellow mosaic disease was characterized by bright yellow mosaic spots on leaves. Amplification of an expected 598 bp DNA band has been done by polymerase chain reaction (PCR) using primers specific to Santiniketan coat protein (AV1) gene (HQ221570.1) of mungbean yellow mosaic India virus (MYMIV). Comparison of sequences obtained from PCR products with corresponding nucleotide sequences in GenBank, confirmed association of causative agent with mungbean yellow mosaic India virus<em>.</em> Nucleotide sequence analyses of MYMIV- Santiniketan isolates of French bean showed high sequence similarities (&gt; 95%) with others isolates present world-wide. A close relationship of coat protein gene isolate of Santiniketan with DNA-A of Pant Nagar, Uttarakhand (DQ389152.1) of mungbean yellow mosaic India virus has been established on phylogenetic tree analysis. This report provides an evidence of MYMIV infections in French bean in Santiniketan.