Alcoholysis of vegetable oil catalyzed by an isozyme of <i style="">Candida rugosa</i> lipase for production of fatty acid esters

Abstract

Celite immobilized Lipase 4, an isoenzyme of commercial <i style="">Candida rugosa</i> lipase (CRL) obtained from the fungus, <i style="">Candida rugosa </i>has been catalyzed alcoholysis in dry ethanol of various triglycerides and soybean oil. The advantages of this process are that separation of the product is very easy and no additional solvent is required as alcohol acts both as a reactant and medium. The yield of monoglycerides affected by temperatures and different organic solvents has been discussed. The efficiency of catalyzed alcoholysis and operation stability of Lipase<i style=""> </i>4 was found to be better than CRL.