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http://nopr.niscpr.res.in/handle/123456789/5598| Title: | Entrapment of lipase in polymer of polyvinyl alcohol-boric acid for esterification in organic media |
| Authors: | Dave, Rachna Madamwar, Datta |
| Keywords: | boric acid;entrapment;ethyl butyrate;lipase;polyvinyl alcohol |
| Issue Date: | Jul-2006 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 C12N9/20; C12N11/08; C12R1/72 |
| Abstract: | Polyvinyl alcohol (PVA)-boric acid method has been utilized for entrapment of Candida rugosa lipase. Immobilized lipase was used to produce ethyl butyrate, a flavour ester showing 80.2% conversion in 72 h. Lipase in PVA-boric acid beads possessed the ability to synthesize variety of esters and was stable in various organic solvents with varying log P value from 2 to 8 under incubation at 50°C for 1 h.The immobilized lipase showed nearly full retention of activity even after 8 cycles of use, the activity then gradually decreased reaching to 56% conversion efficiency after 20 cycles and possesses a shelf-life of 10 months. The thermostability of the lipase increased three times upon immobilization. The immobilized enzyme possessed 40% higher activity compared to its free counterpart. |
| Page(s): | 368-372 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.05(3) [July 2006] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 5(3) 368-372.pdf | 73.72 kB | Adobe PDF | View/Open |
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