Amplified fragment length polymorphism (AFLP) analysis of genetic diversity in Indian mungbean [<i style="">Vigna radiata </i>(L.) Wilczek] cultivars

Abstract

Released cultivars and improved lines of mungbean [<i style="">Vigna radiata </i>(L.) Wilczek] were subjected to AFLP (amplified fragment length polymorphism) analysis to test its usefulness and also to have an assessment of the genetic diversity and relationships among the cultivars. Relative efficiency of the primers, having three (+3) vs. two (+2) selective nucleotides, was also tested for detecting polymorphism. A total of 731 amplification products were obtained in the 27 cultivars with twelve primer pairs. Higher percent polymorphism was obtained with +3 than with +2 primers, though the number of amplification products was much higher with +2 primers. Consequently, higher average similarity coefficient (0.849) was obtained with +2 primers in comparison to +3 primers (0.751). Overall, a narrow genetic diversity (0.681-0.925) was recorded among the cultivars analysed. Distinct clusters were formed in the dendrogram with some variations in the constituents when data from +3 primers alone was compared with that from +2 primers. Principle coordinates analysis supports the results of UPGMA, as there was general agreement between the clustering patterns in both the analyses. The ‘Eigen’ vectors analysis indicated that the contributions of the first three factors were 12.49, 9.44 and 6.71, respectively. Twenty-seven factors were required to explain the total variation observed. Narrow genetic base observed is likely to be due to the use of limited material in the development of the cultivars analysed.