Marker assisted selection and F6 generation characterization of Katarni rice (Oryza sativa L.) derived lines

Abstract

Characterisation of promising progenies in the advanced generations of breeding schemes is essential to know about their distinctness from the parents as well as other existing cultivars. This also requires for developing them as a variety or pre-breeding line. In this study, an attempt was made for establishing distinctness of twenty five F6 families of Katarni x Rajendra Sweta, Katarni x IR64 and Katarni X MTU7029 along with 14 rice genotypes. The genotypes were characterised with the help of thirty simple sequence repeat (SSR) markers. The markers generated 107 polymorphic alleles with an average of 3.56 alleles per locus. The values polymorphic information content (PIC), expected heterozygosity (H) and discriminating power (D) of markers varied from 0.41 to 0.48, 0.32 to 0.50 and 0.75 to 0.96, respectively. At 0.38 similarity coefficient four distinct clusters were formed and cluster I had highest number accessions of F6 families. The study advocated use of (SSR) markers as a complementary tool to morphological descriptors to decipher the distinctiveness of the promising lines in advanced generations of a breeding programme.