Rooting and hardening of<i style=""> in vitro</i> plantlets of <i style="">Garcinia</i> <i style="">indica</i> Chois

Abstract

In the present paper, a procedure for rooting and acclimatization of <i style="">in</i> <i style="">vitro</i> plantlets raised from immature seed cultures of <i style="">Garcinia</i> <i style="">indica</i> is described. Pulse treatment of 9800µ<i style="">M</i> IBA was preferred for rooting of <i style="">in</i> <i style="">vitro</i> shoots. Nearly 100% rooting (<i style="">in</i> <i style="">vitro</i>) was obtained with half strength MS with 2% sucrose and 0.6 % agar. Rooted plantlets of about 2-4 cm height (2-3 nodes) were subsequently transferred to different media for acclimatization: 1. Cocopeat + Sand + Soil (1:2:1); 2. Cocopeat + Sand (1:1); 3. Cocopeat. Plants grown in cocopeat showed 76% of survival followed by cocopeat and sand (1:1) with 75% of survival. The <i style="">in</i> <i style="">vitro</i> elongated shoots could also be rooted <i style="">ex</i> <i style="">vitro</i> by treating with synthetic hormones like Keradix, Rootex-1, and Rootex-3. However, the success of rooting was low (20 to 55 %). The hardened plantlets were successfully acclimatized in the greenhouse and transferred to open field conditions.