Cyclodextrin glycosyl transferases from<i style=""> Bacillus circulance</i> and<i style=""> Bacillus </i>sp.

Abstract

Cyclodextrin glycosyl transferase production and its activity was evaluated using locally isolated <i style="">Bacillus </i>sp. and <i style="">Bacillus circulance</i>. The enzyme production and its induction processes are dependent on the type of the organism. Isolated <i style="">Bacillus </i>sp. possesses higher enzyme productivity and early induction to that of <i style="">B. circulance</i>. The enzyme synthesis in both these strains was observed to be dependent on the carbon source and inoculum level. Activity related experimental results denoted that the properties of the enzyme vary with the source of the organism with respect to hydrogen ion concentration, temperature profile and presence of the ionic species. Overall, the data indicate that both organisms differ in CGTase production and isolated <i style="">Bacillus </i>sp. possesses higher potential as enzyme producer to that of <i style="">B. circulance</i>.