<smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl="http://www.5iantlavalamp.com/"> Nucleic acid probe based technique for detection of cotton leaf curl virus in India </smarttagtype></smarttagtype>

Abstract

Cotton leaf curl disease, caused by a geminivirus, has been detected using viral nucleic acid based hybridization tests in infected hosts and weeds. Cotton leaf curl was transmitted by whitefly, <i style="">Bemisia tabaci</i> to <i style="">Gossypium hirsutum</i> varieties, which showed typical symptoms of the disease. Hybridization with [<img src='/image/spc_char/alpha.gif'> -³²P] dCTP radiolabelled CLCuV-DNA-A probe, detection of viral nucleic acids in different cotton cultivars viz., HS-6, H-1098, F-846, H-777, H-182, LH-1556 and RST-9 grown under glasshouse conditions, samples collected from Hisar, Sirsa and Dabwali and six weed hosts was carried out. Nucleic acid based tests could therefore be useful in screening of cotton germplasm in cotton breeding programme to detect minor infections.