<i style="">In vitro</i> cloning of ornamental species of <i style="">Dianthus</i>

Abstract

<i style="">Dianthus caryophyllus, D. chinensis </i>and <i style="">D. barbatus</i> were micropropagated<i style=""> </i>from shoot tips and nodal segments, which were cultured on MS medium supplemented with BAP and NAA. Best response in terms of multiple shoot formation was observed on MS medium with NAA (0.5 mg/l) + BAP (1 mg/l). In four weeks 10-15 shoots were formed. Every two weeks, inoculum of 6-8 shoots was transferred to a fresh medium for further shoot differentiation. Rooting of <i style="">in vitro</i> shoots was achieved on half strength, MS + IAA (0.5 mg/l). Plantlets that were transferred to pots matured and flowered and resembled seed grown plants Variation of any sort could be avoided as regeneration of shoots was obtained without intervening callusing.