Anti-oxidant activity of compounds and fractions of Manilkara hexandra (Roxb) bark

Abstract

Isolation of a protein mixture having IC50 of 60µg/mL against Streptococcus mutans from the methanol extract of bark of Manilkara hexandra (Roxb) along with six plants phenols Catechin, Catecol, Gallic acid, Phloroglucinol, Quercetin and Rutin has been reported. Where in phenolic constituents of the bark has been reported for the first time and the isolated compounds having moderate activity against S.mutans.In this work, the anti-oxidant activity of these pure compounds and fractions has been studied and its relevance in oral care is established. Comparative anti-oxidant activity of all the six compounds along with the crude extract and fractions have been assessed using eight different assay protocols. The results indicate that twice daily tooth cleaning with the bark can provide basic oral disease protection and thereby assuring several health benefits. Synergistic activity of the phenolic constituents along with the protein and other constituentsmight be a reasonable scientific explanation for the folklore use of the plant as one of the prescription for oral diseases.